Małgorzata Stokrocka, Małgorzata Sznitowska, Maria Żebrowska

Szybkość uwalniania in vitro substancji leczniczej z czopków wyznaczona metodą spektrometrii UV-VIS i pierwszej pochodne

In vitro determination of release rate of active substances from suppositories, by UV-VIS spectrophotometry and first derivate methods. Determination of diclofenac sodium and paracetamol by UV-VIS spectrophotometry is a very simple method, but in the case of pharmaceutical preparations it is badly affected by interferences caused by the excipients accompanying. Spectrophotometry UV (UV-S0) and first derivate spectrophotometry (UV-SI) were compared as methods for the quantitative analysis of the liberation rate of diclofenac sodium and paracetamol from suppositories. Seven different products were analysed and the results obtained with the UV-S0 were generally in a good agreement with those obtained with UV-SI and HPLC. However for two products the difference between the two spectrophotometric methods was larger (up to 10%) what reflects the effect of the excipients dissolved in the dissolution media. It was demonstrated that the UV-SI method is more accurate in such cases.