Katarzyna Winsz-Szczotka, Katarzyna Komosińska-Vassev, Krystyna Olczyk

Wpływ stresu oksydacyjnego na przemiany proteoglikanów tkankowych w przebiegu leczonej tiamazolem choroby Gravesa-Basedowa

Influence of oxidative stress on tissue proteoglycans metabolism in the course of tiamazol treated Graves- disease. In this study we examined whether oxidative stress observed correlate with the metabolic changes of proteoglycans (PGs) in this pathology. Oxidative protein modification, including core proteins of PGs, was assessed by serum thiol group level determinations. The changes of PGs metabolism have been evaluated through the serum GAGs content. GAGs were isolated from blood samples derived from healthy subjects and Graves patients before and after treatment, by the multistage extraction and purification using papaine hydrolysis, alkali elimination, as well as cetylpyridium chloride binding. Total amount of GAGs was quantified by the hexuronic acids assay. Determinations of the serum thiol group level were based on the thiol-disulphide interchange reaction between 5,5-dithiobis (2nitrobenzoic acid) and biological thiols. Increased serum concentration of GAGs in non-treated Graves patients was found. On the contrary, serum thiol group concentrations in untreated patients were significantly decreased. The attainment of euthyreosis was accompanied by the decreased serum GAGs level and by normalization of serum thiol group concentration. We postulate that the alterations of tissue PGs metabolism reflected by quantitative serum GAGs changes, may be connected with excessive reactive oxygen species formation observed in Graves disease patients.