Maciej Małecki, Paweł Swoboda, Katarzyna Banasik, Przemysław Janik
Transdukcja komórek nowotworowych
za pomocą preparatów genowych rAAV
Transduction of cancer cells with rAAV gene preparations. Gene therapy is a therapeutic method based on gene transfer to targeted cells and tissues. The genes are delivered with the carrier commonly recognized as vectors. This work is focused on recombinant adeno-associated virus vector preparations encoding marker gene, namely b-galactosidase (rAAV). rAAV gene preparations were used in vitro experiments performed on ovarian cancer cell line (OVP 10) cells and human embryonic kidney cell line (HEK293) cells. The efficiency of transduction of the cells were evaluated by b-gal test. The results of this study revealed that rAAV vectors have the ability to delivery the gene of interest to studied cells and the efficiency of transduction is mainly depended on virus titer and cell type. The next work will check the possibilities of application of rAAV in the clinic. Pre-clinical data demonstrate the vector safety and efficacy for gene therapy clinical studies.